Multiorgan Development of Oxidative and Nitrosative Stress in LPS-Induced Endotoxemia in C57Bl/6 Mice: DHE-Based In Vivo Approach
Nitric oxide production ex vivo in tissues and nitrite/nitrate burst in plasma in a murine model of endotoxemia, 6 and 12 hours after LPS challenge. EPR spin trapping as described in Materials and Methods was used to detect nitric oxide ex vivo in the aorta, lung, and liver tissues (a, b, and c, respectively), whereas nitrite (d) and nitrate (e) were measured in the plasma using the ENO-20 apparatus. Gradually increased NO production was seen in the lungs at 6 and 12 hours post-LPS injection (b), while the liver showed a much higher NO overproduction (c, 6-7-fold change). Data are presented as or (in c), and statistical significance was tested using one-way ANOVA post hoc LSD’s test or Kruskal-Wallis’s, post hoc Dunn’s (in c), depending on the distribution and homoscedasticity of data. values < 0.05 were considered significant, with <0.05, <0.01, <0.005, and <0.001 versus the control group.
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