|
| Target gene | Primer sequence (5-3) |
|
| IGF1 | Forward 5-GCTTTTGTGATTTCTTGAAGGTGAA-3 |
| Reverse 5-CATACCCTGTAGGCTTACTGAAGTA-3 |
|
| AMPK | Forward 5-CCATCTGTCATTAGTCTTCTG-3 |
| Reverse 5-AGGCTTCGTCATCAATCAT-3 |
|
| PI3K | Forward 5-GTCCTTGAGCCACTGATG-3 |
| Reverse 5-TGTTGCCTTACGGTTGTT-3 |
|
| Akt | Forward 5-AGGAGGAAGAGATGATGGAT-3 |
| Reverse 5-GAATGGATGCCGTGAGTT-3 |
|
| JNK | Forward 5-CAGATAAGCAGTTAGATGAGAG-3 |
| Reverse 5-GACAGATGACGACGAAGAT-3 |
|
| FOXO | Forward 5-CAGCAATGTCAAGGAGAGCA-3 |
| Reverse 5-TGAAGAGGTTGTCCGAGTCC-3 |
|
| IGF1R | Forward 5-GCGTGAGAGGATAGAGTTC-3 |
| Reverse 5-TGTTGGCGTTGAGGTATG-3 |
|
| GLUT1 | Forward 5-TAGTACTGGAGCAGGTGGCAGA-3 |
| Reverse 5-CGGCACAAGAATGGATGAAA-3 |
|
| GLUT3 | Forward 5-TCCCCAGAGCTTCTTACCTCAC-3 |
| Reverse 5-CAGCAAAAGCCAAGACATTCAC-3 |
|
| GLUT8 | Forward 5-CCAAATGGGAACAACTCATCAA-3 |
| Reverse 5-GGGCAAAACCAGCAACAAA-3 |
|
| IGFBP1 | Forward 5-TGGCTCGGGCTAGCTGGATG-3 |
| Reverse 5-ACCAGCACCCAGCGGAATCT-3 |
|
| IGFBP2 | Forward 5-TGTGACAAGCATGGCTTGTACA-3 |
| Reverse 5-TCTCCACGCTGCCCATTC-3 |
|
| IGFBP3 | Forward 5-ATGGTCCCTGTCGTAGAG-3 |
| Reverse 5-ATCCAGGAAGCGGTTGT-3 |
|
| IGFBP4 | Forward 5-TGGTGCGTGGACCGCAAGAC-3 |
| Reverse 5-AGCGATGGGGGCGTCCCATA-3 |
|
| IGFBP5 | Forward 5-TGTGCCTCTGGCAGGGGGTA-3 |
| Reverse 5-CAACACAGCCCACGCTTCCG-3 |
|
| IGFBP7 | Forward 5-TGTGAAGTCATTGGCATCC-3 |
| Reverse 5-CCTCTCCTTTGGCATTTGA-3 |
|
| IR | Forward 5-CAAACGGTGACCAAGCCTCA-3 |
| Reverse 5-CATCCTGCCCATCAAACTCC-3 |
|
| IRS1 | Forward 5-TCCACCACCACCACCATCAC-3 |
| Reverse 5-ACAGCAGCCGCATCCGAAT-3 |
|
| MyoD | Forward 5-CCGCCGATGACTTCTATG-3 |
| Reverse 5-GTTGGTGGTCTTCCTCTTG-3 |
|
| MyoG | Forward 5-AGGCTGAAGAAGGTGAAC-3 |
| Reverse 5-GCTCGATGTACTGGATGG-3 |
|
| Mesp1 | Forward 5-GGTCATCACCCTCCTACA-3 |
| Reverse 5-CCATCTCTGCATCCACAA-3 |
|
| MYF5 | Forward 5-GAGGAGGAGGCTGAAGAA-3 |
| Reverse 5-CGGCAGGTGATAGTAGTTC-3 |
|
| MYF6 | Forward 5-GGAGGAGGCTGAAGAAGA-3 |
| Reverse 5-CTCTCGATGTAGCTGATGG-3 |
|
| GATA4 | Forward 5-TCAGACAAGGAAGCGTAAG-3 |
| Reverse 5-ATGGCAGAGACCGAGAAT-3 |
|
| GATA6 | Forward 5-CCGACCACTTGCTATGAA-3 |
| Reverse 5-TTGCTACAGTCATCTGAGTT-3 |
|
| Nkx2.5 | Forward 5-GACAGAGGAAGAGGAGGAA-3 |
| Reverse 5-CGTTCGCTAGATGGTCTC-3 |
|
| GAPDH | Forward 5-AGAACATCATCCCAGCGT-3 |
| Reverse 5-AGCCTTCACTACCCTCTTG-3 |
|
