Neuroprotective Effect of SCM-198 through Stabilizing Endothelial Cell Function

Figure 4

SCM-198 protected neurons via modulating BMECs in BMEC/neuron coculture system. bEnd.3 treated with SCM-198 and cocultured with SH-SY5Y exhibited protection against OGD/R injury by improving the cell viability and the antioxidative ability. (a) Treatment with SCM-198, especially 1 μM and 10 μM, increased bEnd.3 cell viability in OGD/R irritation. (b) SCM-198 could reduce the LDH leakage in SH-SY5Y cells. (c) SCM-198 decreased the production of MDA in SH-SY5Y cells after OGD/R injury. (d) SCM-198 increased the activity of CAT. (e) SCM-198 reduced cell apoptosis in SH-SY5Y. OGD/R-induced cell apoptosis was determined by TUNEL staining; the result showed that OGD/R obviously increased the number of apoptosis, whereas treatment with SCM-198 reduced cell apoptosis. (f) The quantitative analysis of apoptotic cells was calculated. Values are expressed as . versus control group, versus OGD group ().

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