Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blocking the TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response
Treatment with MRS mitigated acetic acid-induced tissue damage. Mice were administered with MRS at a dose of 1 or 10 ml/kg by gastric gavage for a week before colitis establishment. SASP was used as a positive control at a dose of 500 mg/kg. Colitis was induced by acetic acid solution () injection into the lumen of the colon intrarectally with a volume of 1 ml and dose of 5% except for the sham group. The (a) spleen weight, (b) disease activity index (DAI), (c) colon weight/length ratio, (d) ulcer area, and (e) ulcer index were measured. Additionally, colon tissues were collected to evaluate the damage microscopically by (g) H&E staining (200x) and (f) microscopic scores were calculated. Data were expressed as .,,, and .