Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blocking the TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response
Treatment with MRS improved colonic cell apoptosis caused by acetic acid. Mice were administered with MRS at a dose of 1 or 10 ml/kg by gastric gavage for a week before colitis establishment. SASP was used as a positive control at a dose of 500 mg/kg. Colitis was induced by acetic acid solution () injection into the lumen of the colon intrarectally with a volume of 1 ml and dose of 5% except for the sham group. The collected colon slides were subjected to TUNEL staining to assess cell apoptosis, and green spots represented apoptotic cells (400x). And the apoptosis rate was calculated. Data were expressed as .,,, and .