Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blocking the TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response

Figure 6

Treatment with MRS downregulated the inflammation-related TLR4/NF-κB/MAPK signaling pathway. Mice were administered with MRS at a dose of 10 ml/kg by gastric gavage for a week before colitis establishment. SASP was used as a positive control at a dose of 500 mg/kg. Colitis was induced by acetic acid solution () injection into the lumen of the colon intrarectally with a volume of 1 ml and dose of 5% except for the sham group. Colon tissues were collected, and the protein levels of major pathway components, including TLR4, MyD88, ERK, p-JNK, p38, and NF-κB p65, were analyzed by Western blotting. Data were expressed as . , , , and .