Icariin Inhibits AGE-Induced Injury in PC12 Cells by Directly Targeting Apoptosis Regulator Bax

Figure 2

ICA protected PC12 cells against AGE-induced apoptosis. (a) PC12 cells were exposed to AGEs and treated with ICA (5, 10, and 20 μM) for 48 h. Cell viability was assessed by MTT and expressed relative to control. (b) PC12 cells were treated as in (a), and LDH release was detected with a commercial kit. (c) Apoptotic nuclei were identified using Hoechst 33258 staining. Scale  μm. (d) Apoptotic cells were identified by double staining with AO and EB. The cells which took up both dyes were classified as apoptotic (indicated by arrows in the merged images). Scale  μm. All data were presented as from at least three independent experiments. relative to the control group; , , and relative to the model group.

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