The Sirt1/P53 Axis in Diabetic Intervertebral Disc Degeneration Pathogenesis and Therapeutics

Figure 3

Increased expression of acetyl-p53 and decreased expression of Sirt1 in diabetic NP tissues and high glucose-stimulated NP cells. (a) The expression of acety-p53 and Sirt1 was evaluated by western blotting in NP cells derived from nondiabetic rats and diabetic rats. (b, c) The quantification of acety-p53 and Sirt1 immunoblots. (d, e) The mRNA expression of Sirt1 and p53 was evaluated by western blotting in NP cells derived from nondiabetic rats and diabetic rats. (f) The activity of Sirt1, determined using the Sirt1 assay kit, in NP cells treated with various concentrations of glucose. (g, j) The expression of acetyl-p53, p53, and Sirt1 was evaluated by western blotting in NP cells treated with glucose in a dose-dependent manner and a time-dependent manner. (h, i, k, l) The quantification of immunoblots of acetyl-p53/p53 and Sirt1 is shown. The experiment was repeated at least three times, with a representative example shown. The data in the figures represent the . Significant differences between groups are indicated as , , and .

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