The Sirt1/P53 Axis in Diabetic Intervertebral Disc Degeneration Pathogenesis and Therapeutics

Figure 4

The activation of Sirt1 by butein promotes viability in hyperglycaemic NP cells and suppresses p53 acetylation. (a) Chemical structural formula of butein. (b) Cell viability of NP cells when treated with butein in a dose-dependent manner. (c) Cell viability of NP cells in four groups. (d) The expression of Sirt1 was evaluated by western blot in NP cells treated with butein in a dose-dependent manner. (e) The quantification of Sirt1 immunoblots. (f) The activity of Sirt1 in NP cells in different groups using the Sirt1 assay kit. (g) The expression of ace-p53, p53, and Sirt1 was determined by western blot in NP cells. (h, i) The quantification of Sirt1, ace-p53, and p53 immunoblots. (j) Double labelling immunofluorescence staining of Sirt1 and ace-p53 results in NP cells as treated above and observed in an Olympus fluorescence microscope (original magnification ×400, scale bar: 25 μm). (k) More than three randomly selected images and the quantification of the immunofluorescent intensity of Sirt1 and ace-p53 are shown. The experiment was repeated at least three times independently, with a representative example shown. The data in the figures represent the . Significant differences between groups are indicated as , , and .

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