The Sirt1/P53 Axis in Diabetic Intervertebral Disc Degeneration Pathogenesis and Therapeutics

Figure 6

The activation of Sirt1 suppresses senescence in nucleus pulposus cells. NP cells were treated with DMEM (20% FBS), glucose (50 mM), glucose (50 mM) and butein (10 μM), or glucose (50 mM) and butein (10 μM) plus Ex-527 (10 μM). (a, b) The SA-β-gal staining results of nucleus pulposus cells as treated above are shown (original magnification ×200, scale bar: 50 μm), and the quantification of senescence cells is shown. (c) The expression of senescence markers, such as p16INK4a and p21WAF1, was evaluated by western blot in NP cells as treated above. (d, e) The quantification of p16INK4a and p21WAF1 immunoblots is shown. The experiment was repeated three times independently, with a representative example shown. The data in the figures represent the . Significant differences between groups are indicated as , , and .

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