Nutritional Preconditioning of Apigenin Alleviates Myocardial Ischemia/Reperfusion Injury via the Mitochondrial Pathway Mediated by Notch1/Hes1

Figure 1

The schematic diagram for nutritional preconditioning using Api. (a) Rats were fed with 5 mg/kg body weight/day Api-containing chow daily for 3 weeks and then with standard chow 24 h before establishing the SI/R model. According to the pharmacokinetics theory, after 4-6 half-lives (approximately 4-6 hours), Api is cleared away completely in rats. (b) Cardiomyocytes were pretreated with 20 μM Api for 24 h, and then normally cultured for 19 h prior to SI/R injury.

(a)

(b)