Abnormal Meiosis Initiation in Germ Cell Caused by Aberrant Differentiation of Gonad Somatic Cell

<div>Meiosis was blocked at leptotene stage in both male and female germ cells of <i>Wt1<sup>R394W/R394W</sup></i> gonads. Immunofluorescent staining of SYCP3 (red) and <i>γ</i>H2AX (green) were performed on chromosome spreads of control (<i>Wt1<sup>+/R394W</sup></i>; A–F: female; J–L: male) and <i>Wt1<sup>R394W/R394W</sup></i> (G–I: female; M–O: male) gonads which were dissected at E12.5 and cultured in vitro for 3 days. The gender of the embryos was confirmed with PCR using <i>Sry</i> primers.</div>

Oxidative Medicine and Cellular Longevity

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Figure 7

Figure 7: Abnormal Meiosis Initiation in Germ Cell Caused by Aberrant Differentiation of Gonad Somatic Cell 