Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 3

Research Article

Differential Modulation of NF-κB in Neurons and Astrocytes Underlies Neuroprotection and Antigliosis Activity of Natural Antioxidant Molecules

Figure 3

Effect of antioxidant molecules on neuronal ROS levels and survival. (a) MTT assay of cortical neurons exposed for 24 h to CM-LPS from LPS-stimulated astrocytes. Where indicated, neurons were preincubated ON with RSV (10 μM), QRC (10 μM), ALA (10 μM), CRC (10 μM), LYC (10 μM), OLP (100 μg/ml), GTE (12.5 μg/ml), NAC (300 μM), or pool-R. (b) Quantitation of neuronal ROS levels by FACS analysis of DCH2F-DA fluorescence following exposure to CM from LPS- or TNFα-stimulated astrocytes for 6 or 24 h. (c) Neuronal ROS levels following a 6 h treatment with CM-LPS. Where indicated, neurons were preincubated with the indicated antioxidants or the pool. (d) Survival of cortical neurons following 12 h treatment with H2O2 (200 μM) or Glut (200 μM), in the presence/absence of the indicated antioxidants or the lower concentration pool. (e) Time course of neuronal ROS production during incubation with H2O2 (200 μM) or Glut (200 μM). (f) Neuronal ROS levels following treatment with H2O2 (200 μM) or Glut (200 μM). Where indicated, neurons were preincubated with the indicated antioxidants or the pool. Data are expressed as percent of CTR. MTT data are the of three independent experiments, each performed with 4-6 samples for each treatment. ROS data are the of three experiments in duplicate. , , and versus CTR (ANOVA and Dunnett’s multiple comparison test).
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