Differential Modulation of NF-<i>κ</i>B in Neurons and Astrocytes Underlies Neuroprotection and Antigliosis Activity of Natural Antioxidant Molecules

<div>Modulation of neuronal transcription factor activity by glial activation and RSV. (a) Protein-DNA array of nuclear extracts prepared from cortical neurons treated for 2 h with CM (from LPS-stimulated astrocytes), RSV (10 <i>μ</i>M), or CM-LPS plus RSV. The grid for location of transcription factors (<a href="https://www.biocat.com/bc/pdf/MA1210_MA1215_Protein_DNA_Arrays">https://www.biocat.com/bc/pdf/MA1210_MA1215_Protein_DNA_Arrays</a>). (b) Quantitation of transcription factors. (c) Bar graph for quantitative analysis of the ratio RSV/CTR and CM-LPS+RSV/CM-LPS. Light grey square = no change or reduction versus CTR; dark grey square = induction versus CTR (<span class="nowrap"><svg height="9.9449pt" id="M38" style="vertical-align:-0.6369991pt" version="1.1" viewbox="-0.0498162 -9.3079 52.6947 9.9449" width="52.6947pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g><g transform="matrix(.013,0,0,-0.013,9.872,0)"><path d="M512 230V281L75 514V456L453 256V254L75 55V-3L512 230Z"></path></g><g transform="matrix(.013,0,0,-0.013,21.135,0)"><path d="M412 140C382 77 369 73 315 73H129L270 222C362 320 402 379 402 466C402 571 322 635 234 635C177 635 130 609 99 576L42 495L64 475C90 514 133 568 201 568C274 568 318 519 318 435C318 349 255 267 193 193C144 135 87 78 32 23V0H405C417 45 427 89 440 131L412 140Z"></path></g><g transform="matrix(.013,0,0,-0.013,27.375,0)"><path d="M300 253H71L57 198H286L300 253Z"></path></g><g transform="matrix(.013,0,0,-0.013,32.02,0)"><path d="M54 437L27 408L31 397H101V103C101 37 94 32 30 26V0H266V25C187 33 180 36 180 110V397H288C299 404 304 428 298 437H180V477C179 562 190 610 203 630C214 647 230 659 256 659C289 659 318 641 337 622C346 612 355 612 364 619C374 627 380 635 383 643C388 655 387 667 378 678C362 697 333 710 299 712C260 707 225 689 189 659C135 613 119 563 112 541S101 490 101 458V437H54Z"></path></g><g transform="matrix(.013,0,0,-0.013,35.751,0)"><path d="M257 449C165 449 37 374 37 209C37 98 119 -12 256 -12C355 -12 473 65 473 226C473 349 381 449 257 449ZM244 416C333 416 380 320 380 204C380 67 329 21 267 21C184 21 130 115 130 241C130 354 184 416 244 416Z"></path></g><g transform="matrix(.013,0,0,-0.013,42.289,0)"><path d="M238 0V26C174 32 166 38 166 104V712C132 700 70 683 18 677V653C81 647 87 645 87 577V104C87 38 78 32 15 26V0H238Z"></path></g><g transform="matrix(.013,0,0,-0.013,45.578,0)"><path d="M517 51L485 54C448 58 441 63 441 115V712C404 700 337 684 285 678V653C357 648 362 645 362 580V437C339 446 309 449 295 449C159 449 38 340 38 201C38 61 143 -12 223 -12C234 -12 261 -6 301 17L362 53V-12C420 9 495 22 517 26V51ZM362 85C338 67 301 51 266 51C201 51 128 109 128 228C128 373 212 411 259 411C296 411 338 395 362 360V85Z"></path></g></svg>);</span> grey square = induction versus CTR (&gt;2‐fold).</div>

Oxidative Medicine and Cellular Longevity

fig6

Figure 6

Figure 6: Differential Modulation of NF-<i>κ</i>B in Neurons and Astrocytes Underlies Neuroprotection and Antigliosis Activity of Natural Antioxidant Molecules 

Figure 6 | Differential Modulation of NF-κB in Neurons and Astrocytes Underlies Neuroprotection and Antigliosis Activity of Natural Antioxidant Molecules 