Hydrogen Sulfide Gas Exposure Induces Necroptosis and Promotes Inflammation through the MAPK/NF-κB Pathway in Broiler Spleen

Figure 4

The ATPase activities and energy metabolism-related expressions in chicken spleens. The effect of H₂S on energy metabolism of chicken spleens. (a) represents the activities of Na⁺-K⁺-ATPase, Ca²⁺-Mg²⁺-ATPase, Ca²⁺-ATPase, and Mg²⁺-ATPase of spleen tissues. (b) and (c) represent the mRNA and protein expressions of spleen tissues, respectively. The results are from at least three independent experiments. Data are represented as the (). β-Actin and GAPDH were selected as the reference of mRNA and protein expressions, respectively. shows significant difference from the corresponding control groups (); shows significant difference from the corresponding control groups ().

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