Hydrogen Sulfide Gas Exposure Induces Necroptosis and Promotes Inflammation through the MAPK/NF-κB Pathway in Broiler Spleen

Table 1

mRNAs primer sequences.

Gene	Forward 5 to 3	Reverse 5 to 3
RIPK1	AAGGGCGTTTCATCCTGGAG	CGGCAGGTCTCTTCTTTGGT
RIPK3	CCCATGGACAGGGAATGGAA	CCACAAGTCTCTGGTAGCGG
MLKL	CCATGGGTGGTTCCTCCTTC	TGGATCTTCCGCACCTTAGC
Caspase-8	CCGATTCTCTGGGCAACTGT	ATCCACATGTGTCCCGTTCC
JNK	TGACCGAGTGAGGAGACGAT	ACTGTATCGAACGCAGCACA
ERK	AGAATCTCACAGCGTCTCGC	GGTGTGATTCATCAGCATCTTCA
p38	GCGAGTCCCTAATGCCTACG	ACAACTGTTGAGCCACACTCA
TAK1	ACCGGGTTAAACGGATCCAC	TCGTTTTGCTCGTGCTTTGG
TAB2	CTCTTTTTCCTTGGCGAGCG	GCTTCCTTGGGCCATTCGTA
TAB3	TTGAACCACCGCAAAGACCT	GGTTTGGGTTGACCCGACAT
TNF-α	GCCCTTCCTGTAACCAGATG	ACACGACAGCCAAGTCAACG
IL-1β	ACTGGGCATCAAGGGCTACA	GCTGTCCAGGCGGTAGAAGA
NF-κB	TCAACGCAGGACCTAAAGACAT	GCAGATAGCCAAGTTCAGGATG
HSP70	CGGGCAAGTTTGACCTAA	TTGGCTCCCACCCTATCTCT
HSP90	TCCTGTCCTGGCTTTAGTTT	AGGTGGCATCTCCTCGGT
NOX2	GGACTGTCCATCTTTGTCGT	TTACACGGGTAGAGCAGCAC
PK	AGCAGCAGGAGACACCGAAC	TGAGGCGGGCAACATTCAT
HK2	TTCGACCACATCGTCCACTG	ACCACGTCCAGGTCAAACTC
SDHB	CGGTCCAGGGGATCTGTCG	AGATGCCTTCCCTGCATGAC
PDHX	ACGCTTGGGCTCCCTAATTG	TCCACTTTAGGAGGGGCAGA
avUCP	GATGCAGAGAAACAGAGCGG	AAGGTGCAGAGGTCAGCGAT
β-Actin	CCGCTCTATGAAGGCTACGC	CTCTCGGCTGTGGTGGTGAA