<i>Pueraria lobata</i> and Daidzein Reduce Cytotoxicity by Enhancing Ubiquitin-Proteasome System Function in SCA3-iPSC-Derived Neurons

<div>Elevated cytotoxicity and caspase 3 expression/activity in MG132-treated iPSC-derived neurons. (a) Lactic dehydrogenase (LDH) release in culture media was measured to evaluate cytotoxicity. LDH level of untreated neurons was normalized as 100%. (b) Western blot analysis of the cleaved caspase 3 level (normalized to TUJ1 as the internal control). Cleaved caspase 3 level of 2 <i>μ</i>M MG132-treated NC-iPSC-derived neurons (NC-1 and NC-2) was set as 100%. (c) Caspase 3 activity assay of NC- and SCA3-iPSC-derived neurons. Caspase 3 activity of untreated neurons was normalized as 100%. Each experiment for each sample was performed in triplicate. <svg height="10.2124pt" id="M25" style="vertical-align:-3.42943pt" version="1.1" viewbox="-0.0498162 -6.78297 7.83752 10.2124" width="7.83752pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M570 304C570 398 525 448 414 448C385 448 343 445 312 434L329 511L321 518C297 504 262 482 244 460L233 411C195 397 159 381 128 358L135 332C160 347 189 360 224 373L111 -147C97 -210 84 -218 17 -231L13 -257L254 -247L259 -218L233 -216C183 -212 177 -202 189 -142L218 -1C238 -10 266 -12 283 -12C351 3 429 48 483 105C543 168 570 242 570 304ZM482 289C482 161 380 33 304 33C278 33 248 51 233 69L303 396C326 400 352 403 369 403C428 403 482 380 482 289Z"></path></g></svg> values: MG132-treated vs. untreated, <span class="nowrap"><svg height="13.532pt" id="M26" style="vertical-align:-3.4295pt" version="1.1" viewbox="-0.0498162 -10.1025 50.6052 13.532" width="50.6052pt" 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: <i>Pueraria lobata</i> and Daidzein Reduce Cytotoxicity by Enhancing Ubiquitin-Proteasome System Function in SCA3-iPSC-Derived Neurons 

Oxidative Medicine and Cellular Longevity

Pueraria lobata and Daidzein Reduce Cytotoxicity by Enhancing Ubiquitin-Proteasome System Function in SCA3-iPSC-Derived Neurons

Figure 5