Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 1

Research Article

Modulations of Histone Deacetylase 2 Offer a Protective Effect through the Mitochondrial Apoptosis Pathway in Acute Liver Failure

Figure 1

CAY10683 inhibited the mitochondrial apoptotic pathway in the TNF-α/D-gal-treated LO2 cells. (a) The mRNA level of bcl2 and bax mRNA levels were detected by RT-PCR. (b-g) The protein expressions of HDAC2, cyt c in mitochondrion, cyt c in cytosol, caspase 3, c-caspase 3, caspase 9, c-caspase 9, apaf1, bcl2, and bax were detected by western blot. (h) The staining with Annexin V-phycoerythrin/7-aminoactinomycin D (Annexin V-PE/7AAD) and flow cytometry were performed to detect the influence LO2 cell apoptosis. , compared with the control group. , compared with the TNF-α/D-gal-treated model group. c-caspase3: cleaved-caspase 3, c-caspase9: cleaved-caspase 9.

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