Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 3

Research Article

Modulations of Histone Deacetylase 2 Offer a Protective Effect through the Mitochondrial Apoptosis Pathway in Acute Liver Failure

Figure 3

The levels of mitochondrial apoptosis signaling molecules in LO2 cells were increased after HDAC2 overexpression. (a) The HDAC2 overexpression lentiviral vector was made and transfected into LO2 cells. GFP was observed with a fluorescence microscope after 72 h. (b) At 72 h after transfection, the HDAC2 mRNA level was detected by RT-PCR. (c) The mRNA level of bcl2 and bax mRNA levels were detected by RT-PCR. (d-f) The protein expressions of HDAC2, cyt c in mitochondrion, cyt c in cytosol, caspase 3, c-caspase 3, caspase 9, c-caspase 9, apaf1, bcl2, and bax were detected by western blot. (g) The staining with Annexin V-PE/7AAD and flow cytometry were performed to detect the influence LO2 cell apoptosis. , compared with the control group. , compared with the control group. , compared with the TNF-α/D-gal treated model group.

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