Melatonin Reduces Androgen Production and Upregulates Heme Oxygenase-1 Expression in Granulosa Cells from PCOS Patients with Hypoestrogenia and Hyperandrogenia

Table 2

The primer sequences.

Gene (accession no.)	Primer sequence	Product size (bp)
CYP19A1 (DQ118405.1)	5 GGACCCCTCATCTCCCACG 3	195
	5 CCCAAGTTTGCTGCCGAAT 3
SOD1 (NM_000454.4)	5 TGAAGGTGTGGGGAAGCATTA 3	131
	5 ACCGTGTTTTCTGGATAGAGGAT 3
GPx (D00632.1)	5 CCATTCGGTCTGGTCATTCTG 3	107
	5 CCACCTGGTCGGACATACTTG 3
CAT (NM_001752.3)	5 ACCCCTCCTGGACTTTTTACATC 3	115
	5 GGGATGAGAGGGTAGTCCTTGTG 3
HO-1 (NM_002133.2)	5 ATCCCCTACACACCAGCCAT 3	205
	5 CAATGTTGGGGAAGGTGAAGA 3
NOX2 (NM_000397.3)	5 TTGTCAAGTGCCCAAAGGTGT 3	175
	5 TTAGGTAGTTTCCACGCATCTTG 3
Bax (KJ890756.1)	5 ACGGCAACTTCAACTGGGG 3	237
	5 GCACTCCCGCCACAAAGAT 3
Bcl-2 (NM_000633.2)	5 CTTCTTTGAGTTCGGTGGGGT 3	196
	5 CCAGGAGAAATCAAACAGAGGC 3
β-Actin (HQ154074.1)	5 TGCCCTGAGGCTCTTTTCC 3	117
	5 GGCATACAGGTCTTTGCGGAT 3