Research Article
FA-97, a New Synthetic Caffeic Acid Phenethyl Ester Derivative, Protects against Oxidative Stress-Mediated Neuronal Cell Apoptosis and Scopolamine-Induced Cognitive Impairment by Activating Nrf2/HO-1 Signaling
Figure 1
Effect of FA-97 on H2O2-induced cytotoxicity in SH-SY5Y and PC12 cells. SH-SY5Y and PC12 cells were plated in a 96-well plate, treated with H2O2 (500 μM) and FA-97 (0, 0.25, and 0.5, 1 μM) for 24 h. (a) Synthesis scheme of FA-97. FA-97 is synthesized via the coupling reaction between an acetyl-protected brominated D-glucose and caffeic acid phenethyl ester (CAPE) starting from commercially available caffeic acid. (b) Morphological changes in SH-SY5Y and PC12 cells were observed by phase contrast microscopy. (c, d) The viability of SH-SY5Y (c) and PC12 cells (d) was tested by CCK8 assay. (e, f) Effects of FA-97 on the released LDH of SH-SY5Y (e) and PC12 cells (f) induced by H2O2 were detected. Data from three times independent experiments were expressed as . and compared with the control group and and compared with the H2O2-treated group.
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