FA-97, a New Synthetic Caffeic Acid Phenethyl Ester Derivative, Protects against Oxidative Stress-Mediated Neuronal Cell Apoptosis and Scopolamine-Induced Cognitive Impairment by Activating Nrf2/HO-1 Signaling

Figure 5

Nrf2 is involved in the antioxidant effect of FA-97 on neuronal cells. Nrf2 siRNA and nontargeting siRNA (NT siRNA) were transfected into SH-SY5Y cells. After being cultured in serum-free medium for 8 h, cells were treated with H₂O₂ (500 μM) and FA-97 (0, 0.25, 0.5, and 1 μM) for 24 h. (a) The expression of Nrf2 was detected by Western blot assay. (b) The relative ratio of Nrf2 was represented by densitometric analysis. (c) The viability of SH-SY5Y cells was evaluated by CCK8 assay. (d) SH-SY5Y cells were incubated with DCFH-DA for 30 min at 37°C in the dark, and then the ROS level was measured by spectrofluorometer. (e) The total antioxidant capacity of SH-SY5Y cells was detected according to the kit manufacturer’s instruction. (f) The expression of HO-1, NQO-1, and β-actin was measured by Western blot analysis. (g) The relative ratios of HO-1 and NQO-1 were represented by densitometric analysis. The results are representative of three independent experiments and expressed as . and compared with the control group; compared with the H₂O₂+FA-97-treated group.

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