Plant Sterol Ester of α-Linolenic Acid Attenuates Nonalcoholic Fatty Liver Disease by Rescuing the Adaption to Endoplasmic Reticulum Stress and Enhancing Mitochondrial Biogenesis

Figure 2

PS-ALA alters protein expression of gene involved in ER stress. Effect of PS-ALA on mRNA expression of (a) Grp78 and (b) Chop was assayed with quantitative real-time RT-PCR. Relative mRNA expression is shown as a ratio relative to β-actin and reported as (). ^a versus the control group; ^b versus the HFD group. (c) The effect of PS-ALA on protein expression of Grp78 and Chop in the liver was assayed by western blotting, with β-actin as a loading control. Representative images of at least three independent experiments are shown. Protein expression of (d) Grp78 and (e) Chop is presented as fold change relative to the control. Each bar denotes the (). ^a versus the control group; ^b versus the HFD group.

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