Protective Effect of Ginsenoside Rg1 on Oxidative Damage Induced by Hydrogen Peroxide in Chicken Splenic Lymphocytes

Figure 4

Effect of Rg1 on mitochondrial depolarization. Cells () were treated with Rg1 (0, 50, 70, and 90 μg/mL) for 24 h first and then incubated in media with (model) or without (control) H₂O₂ (100 μmol/L) for an additional 4 h. After that, cells () were incubated with JC-1 (5 μg/mL) and assayed by FCM. Mitochondrial depolarization was presented by a reduction in the red/green fluorescence intensity ratio: (a) control group; (b) model group; (c) 50 μg/mL Rg1 group; (d) 70 μg/mL Rg1 group; (e) 90 μg/mL Rg1 group; (f) bar diagram representing JC-1 polymer/monomer MFI. All data are presented as (). Bars with different letters were significantly different ().

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