Research Article
Protective Effect of Ginsenoside Rg1 on Oxidative Damage Induced by Hydrogen Peroxide in Chicken Splenic Lymphocytes
Figure 5
Effects of Rg1 on H2O2-induced changes in the [Ca2+]i levels. Cells () were treated with Rg1 (0, 50, 70, and 90 μg/mL) for 24 h first and then incubated in media with (model) or without (control) H2O2 (100 μmol/L) for an additional 4 h. After incubation with Fluo-3/AM ([Ca2+]i probe), cells were directly observed under a microscope and Fluo-3/AM fluorescence was measured by FCM: (a) control group; (b) model group; (c) 50 μg/mL Rg1 group; (d) 70 μg/mL Rg1 group; (e) 90 μg/mL Rg1 group; (f) bar diagram representing Fluo-3 MFI. All data are presented as (). Bars with different letters were significantly different ().
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |