[Retracted] Proanthocyanidins Antagonize Arsenic-Induced Oxidative Damage and Promote Arsenic Methylation through Activation of the Nrf2 Signaling Pathway

Figure 10

Western blot analysis of proteins after treatment with arsenic and/or grape seed procyanidin extract. Nrf2, NQO1, HO-1, and GST protein expression was measured in L-02 cells treated with arsenic and/or grape seed procyanidin extract by western blotting. Treatment with GSPE (25 and 50 mg/L) significantly elevated the protein contents of Nrf2, NQO1, HO-1, and GST in a dose-dependent manner compared with the control group. Compared with the control group, arsenic (25 μM) markedly decreased the protein expression of Nrf2 and its downstream genes after 24 h. However, the protein contents of Nrf2, NQO1, HO-1, and GST in the arsenic + GSPE group increased gradually compared with the contents in the arsenic group.