Oleuropein-Induced Apoptosis Is Mediated by Mitochondrial Glyoxalase 2 in NSCLC A549 Cells: A Mechanistic Inside and a Possible Novel Nonenzymatic Role for an Ancient Enzyme

Figure 4

OP-induced mGlo2 upregulation is dependent on p38 MAPK and Akt signaling pathways. (a) Protein level of total Akt (tAkt) phospho-Akt (p-Akt) by western blot (WB) analysis after oleuropein (OP) treatment for 24 hours, (b, d) mitochondrial Glo2 expression, and (c, e) apoptosis, evaluated by WB and active caspase-3 expression, respectively, in A549 cells pretreated with the selective Akt inhibitor MK2206 (MK) (b, c) or the Akt activator SC79 (d, e) and following OP exposure (150 μM). Western blots were stripped of the bound Abs and reprobed with anti-β-actin or Cox-IV, to confirm equal loading. The western blots shown are the representative of three separate experiments. Histograms indicate the of three different cultures each of which was tested in triplicate. (-) untreated and (+) treated cells; , , and versus unexposed cells; ° versus OP-treated cells.

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