Plin5/p-Plin5 Guards Diabetic CMECs by Regulating FFAs Metabolism Bidirectionally

<div>The influence of p-Plin5 on CMECs injury induced by HG-HFFAs. (a) The protein level of Plin5 detected by western blot. (b) The intracellular FFAs content measured by Free Fatty Acid Quantitation Kit. (c) The content of LDs in CMECs determined by Nile red staining. (d) The apoptosis rate. (e) The production of NO measured by ELISA kit. (f) NO generation detected by staining with DAF-2DA in CMECs. Vel: vehicle; ISO: isoproterenol; HG-HFFAs: high glucose and high free fatty acids; ELISA: enzyme-linked immunosorbent assay. 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Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: Plin5/p-Plin5 Guards Diabetic CMECs by Regulating FFAs Metabolism Bidirectionally 