Overexpression of BRCA1 in Neural Stem Cells Enhances Cell Survival and Functional Recovery after Transplantation into Experimental Ischemic Stroke
OGD/R induced NSCs apoptosis and triggered BRCA1 expression. The cultured NSCs were subjected to OGD for 4 h and subsequent reoxygenation for 2 h or 24 h. (a) Cell viability at the indicated times was detected by CCK-8. (b, c) Western blot and quantitative analysis of cleaved caspase-3 and BRCA1 in treated NSCs. (d) Immunofluorescence staining of BRCA1 in control and OGD/R NSCs. Data are expressed as ;.; vs. the control group. Scale bar: 50 μm.