Transcription Factor p53 Suppresses Tumor Growth by Prompting Pyroptosis in Non-Small-Cell Lung Cancer

Figure 2

Overexpression of p53 elevates pyroptotic level in A549 NSCLC cells. A549 lung cancer cells were transfected with p53 overexpression or negative control vector (pcDNA3.1). (a) p53 protein expression level was determined by western blotting analysis; , . (b) p53 mRNA level was determined by real-time PCR; , . (c) Pyroptotic factor mRNA levels were determined by real-time PCR; , , versus the negative control group. (d, e) Pyroptotic proteins were determined by western blotting, and statistical results were obtained by densitometric analysis and normalization to GAPDH; , .

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