Research Article
Transcription Factor p53 Suppresses Tumor Growth by Prompting Pyroptosis in Non-Small-Cell Lung Cancer
Figure 3
Knockdown of p53 inhibits pyroptotic level in A549 NSCLC cells. (a) p53 mRNA level was determined by real-time PCR. A549 lung cancer cells were transfected with siRNA p53 or negative control vector (NC); , . (b) p53 protein expression level was determined by western blotting analysis; , . (c) Pyroptotic factor mRNA levels were determined by real-time PCR; , , versus the NC group. (d, e) Pyroptotic proteins were determined by western blotting, and statistical results were obtained by densitometric analysis and normalization to GAPDH; , .
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