Transcription Factor p53 Suppresses Tumor Growth by Prompting Pyroptosis in Non-Small-Cell Lung Cancer

Figure 4

p53-activated pyroptosis suppresses A549 lung cancer cell proliferation. (a) Pyroptotic factor (NLRP3, ASC, caspase-1, and cleaved caspase-1) mRNA levels were determined by real-time PCR; , , . (b) Immunofluorescent staining showed the effect of LPS on NLRP3-formed inflammasomes (red) in A549 lung cancer cells. Cell nuclei were visualized by DAPI (blue). Scale bar equals 10 μm. (c, d) p53 protein and mRNA levels were determined by western blotting and real-time PCR, respectively; , . (e) p53 protein levels were determined by western blotting, , normalized to GAPDH, , , . (f) Cell viability was detected by CCK-8 assay in A549 cells; , , .

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