Ferritinophagic Flux Activation in CT26 Cells Contributed to EMT Inhibition Induced by a Novel Iron Chelator, DpdtpA

Figure 10

DpdtpA induced alteration in lysosomal membrane permeability and release of cathepsin D. (a) DMSO; (b) 0.78 μM DpdtpA; (c) 1.56 μM DpdtpA; (d) 0.78 μM DpdtpA+3-MA (5 mM); (e) 1.56 μM DpdtpA+3-MA (5 mM); (f) 3-MA (5 mM); (g) quantitative analysis of alteration in fluorescence after treated by either DpdtpA or combined with 3-MA; (h) DpdtpA induced cathepsin D release. The quantification analysis of intensity of red fluorescence was from three measurements. The Western blots were performed twice (^{, ###, $$$}).

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