Ferritinophagic Flux Activation in CT26 Cells Contributed to EMT Inhibition Induced by a Novel Iron Chelator, DpdtpA

Figure 6

DpdtpA induced alteration in morphology correlated with EMT modulation. (a-c) Alteration in morphology treated by DpdtpA for 48 h at indicated concentration; the blue arrow: spindle-shaped cells, black arrow: retracted and rounded cells; (a) 0.7% DMSO; (b) 0.26 μM DpdtpA; (c) 0.52 μM DpdtpA; objective size: , scale bar: 200 μm. (d) Western blotting analysis. (e-l) Immunofluorescence analysis of epithelial-mesenchymal markers. (e-h) (0.7% DMSO): (e) nuclei in blue; (f) E-cadherin in green; (g) vimentin in red; (h) merge of nuclei, E-cadherin, and vimentin in the DMSO group. (i-l) DpdtpA-treated group: (i) nuclei in blue; (j) E-cadherin in green; (k) vimentin in red; (l) merge of nuclei, E-cadherin and vimentin in the DpdtpA-treated group. The measurements were performed thrice from different field of view. Objective size: (fluorescence), scale bar: 100 μm.

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