miR-154-5p Functions as an Important Regulator of Angiotensin II-Mediated Heart Remodeling

Figure 5

miR-154-5p is involved in AngII-induced cardiac remodeling mediated by the AT1 receptor in vivo and in vitro. (a) Representative images of mouse hearts and the ratio of heart weight to body weight (HW/BW) in control (Con), telmisartan- (Tel-), AngII-, and AngII+telmisartan- (AngII+Tel-) treated mice (). (b) Histological analysis of the surface area of cardiomyocytes in the Con, Tel-, AngII-, and AngII+Tel-treated groups using WGA staining (). (c) Images of Masson’s trichrome-stained sections of the hearts from Con, Tel-, AngII-, and AngII+Tel-treated mice (). (d) TUNEL staining and quantitative analysis of sections of the hearts from Con, Tel-, AngII-, and AngII+Tel-treated mice (). (e) Expression of markers of cardiac remodeling in the myocardium of different groups of treated mice, as measured using real-time PCR (). (f) Western blot and quantitative analysis of cleaved caspase-3 and caspase-3 levels in the hearts of Con, Tel-, AngII-, and AngII+Tel-treated mice (). (g) Cardiac expression of miR-154-5p, as detected using real-time PCR (). compared with the Con group; ^# compared with the AngII group. (h) Expression of markers of cardiac hypertrophy in cardiomyocytes treated with GFP+DMSO (G+D), GFP+telmisartan (G+T), GFP+AngII+DMSO (G+A+D), GFP+AngII+telmisartan (G+A+T), miR-154-5p+AngII+DMSO (154+A+D), or miR-154-5p+AngII+telmisartan (154+A+T) (). (i) Western blots showing the levels of cleaved caspase-3 and caspase-3 (). (j) Expression of miR-154-5p in cells subjected to different treatments, as measured using real-time PCR (). , , , and ns = not significant.

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