Metformin Protects against Oxidative Stress Injury Induced by Ischemia/Reperfusion via Regulation of the lncRNA-H19/miR-148a-3p/Rock2 Axis

Figure 4

Expression of lncRNA-H19, Rock2, and miR-148a-3p following ischemia both in vivo and in vitro. (a) RT-PCR assay for the expression of lncRNA-H19 and miR-148a-3p in vivo. (b) Expression of lncRNA-H19 and miR-148a-3p at each time point in N2a cells subjected to 4 h OGD and at 0 h, 6 h, 12 h, 24 h, and 48 h of reperfusion ( per group). (c) Pearson’s correlation analysis of the relationship between lncRNA-H19 and miR-148a-3p in the brain of mice subjected to MCAO/R surgery. RT-PCR assay for the expression of lncRNA-H19 (d), miR-148a-3p (e), and Rock2 (f) in mice subjected to MCAO/R ( per group). and versus the sham group by 1-way analysis of variance with Tukey’s multiple comparison test. ^# versus the MCAO/R group by 1-way analysis of variance with Tukey’s multiple comparison test. RT-PCR was used to detect the expression of lncRNA-H19 (g), miR-148a-3p (h), and Rock2 (i) in N2a cells subjected to OGD/R ( per group). Data are represented as . and versus the control group by 1-way analysis of variance with Tukey’s multiple comparison test. ^# versus the mock group by 1-way analysis of variance with Tukey’s multiple comparison test.

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