Metformin Protects against Oxidative Stress Injury Induced by Ischemia/Reperfusion via Regulation of the lncRNA-H19/miR-148a-3p/Rock2 Axis

Figure 5

miR-148a-3p competitively binds to lncRNA-H19 and Rock2. (a) The lncRNA-H19 was predicted as a target of miR-148a-3p by online bioinformatic methods (Target Scan and http://microrna.org). (b) The luciferase activity of lncRNA-H19 was declined in N2a cells cotransfected with lncRNA-H19-3UTR-WT and miR-148a-3p mimic. (c) The expression level of miR-148a-3p was determined after transfection of siRNA-NC, siRNA-H19, pcDNA3.1-NC, and pcDNA3.1-H19. (d) The mRNA expression of lncRNA-H19 was determined in N2a cells cotransfected with NC, NC mimic, miR-148a-3p mimic, NC inhibitor, and miR-148a-3p inhibitor. (e) The miR-148a-3p was predicted as a target of Rock2 by online bioinformatic methods (Target Scan and http://microrna.org). (f) The luciferase activity of Rock2 was declined in N2a cells cotransfected with Rock2-3UTR-WT and miR-148a-3p mimics. (g–i) The mRNA and protein expression of Rock2 was elevated in N2a cells cotransfected with NC, NC mimic, miR-148a-3p mimic, NC inhibitor, and miR-148a-3p inhibitor. Data are represented as . and versus the NC mimic group by 1-way analysis of variance with Tukey’s multiple comparison test. ^# and ^## versus the NC inhibitor group by 1-way analysis of variance with Tukey’s multiple comparison test.

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