Metformin Protects against Oxidative Stress Injury Induced by Ischemia/Reperfusion via Regulation of the lncRNA-H19/miR-148a-3p/Rock2 Axis

Figure 8

Metformin protects against oxidative stress injury induced by I/R via regulation of the lncRNA-H19/miR-148a-3p/Rock2 axis. (a) The mRNA expressions of lncRNA-H19 were determined after lateral ventricular injection of pcDNA3.1-NC and pcDNA3.1-H19. (b) The level of miR-148a-3p. (c) Statistical analysis of the percentage of infarct volume was determined for each group. (d) Neurological scores after transient middle cerebral artery occlusion (tMCAO) in each groups. (e) Activities of SOD in the serum and the brain. (f) Levels of MDA in the serum and the brain. (g) The mRNA expressions of Rock2, eNOS, HO-1, and Nrf2 were determined after lateral ventricular injection of pcDNA3.1-NC and pcDNA3.1-H19. (h, i) The protein expressions of Rock2, eNOS, HO-1, and Nrf2 were determined after lateral ventricular injection of pcDNA3.1-NC and pcDNA3.1-H19. , , and versus the sham group by 1-way analysis of variance with Tukey’s multiple comparison test. ^#, ^##, and ^### versus the MCAO/R group by 1-way analysis of variance with Tukey’s multiple comparison test. ^&, ^&&, and ^&&& versus the MCAO/R+Met+NC group by 1-way analysis of variance with Tukey’s multiple comparison test.

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