Metformin Protects against Oxidative Stress Injury Induced by Ischemia/Reperfusion via Regulation of the lncRNA-H19/miR-148a-3p/Rock2 Axis

Table 1

List of primers used for real-time RT-PCR.

Primer	Symbol	Sequence (5-3)
Long-chain noncoding RNA H19	lncRNA-H19	Fwd: GTCAGGACCGTGTTCTCAAGG
		Rev: GCTTCTTTGATGTTACTGAGGGC
Rho-associated protein kinase 2	Rock2	Fwd: TTGGTTCGTCATAAGGCATCAC
		Rev: TGTTGGCAAAGGCCATAATATCT
Endothelial nitric oxide synthase	eNOS	Fwd: GGCTGGGTTTAGGGCTGTG
		Rev: CTGAGGGTGTCGTAGGTGATG
Heme oxygenase-1	HO-1	Fwd: AAGCCGAGAATGCTGAGTTCA
		Rev: GCCGTGTAGATATGGTACAAGGA
Nuclear factor erythroid-2-related factor 2	Nrf2	Fwd: CTGAACTCCTGGACGGGACTA
		Rev: CGGTGGGTCTCCGTAAATGG
MicroRNA 148a-3p	miR-148a-3p	Fwd: AGCAGTTCAGTGCACTACAG
		Rev: GCAGGGTCCGAGGTATTC
U6	U6	Fwd: GCGCGTCGTGAAGCGTTC
		Rev: GTGCAGGGTCCGAGGT
Glyceraldehyde-3-phosphate dehydrogenase	GAPDH	Fwd: CACTCACGGCAAATTCAACGGCA
		Rev: GACTCCACGACATACTCAGCAC