Fenofibrate Improved Interstitial Fibrosis of Renal Allograft through Inhibited Epithelial-Mesenchymal Transition Induced by Oxidative Stress

Figure 1

H₂O₂ treatment induced oxidative stress in rat renal tubular epithelial cells. (a) NRK-52e cells were treated with 100 μmol/L H₂O₂ for 0.5 h, 1 h, 1.5 h, 2 h, and 2.5 h, and cell viability was detected with CCK8. (b) NRK-52e cells were treated with 100 μmol/L H₂O₂ for 1 h and 2 h; relative ROS levels were detected with the DCFH-DA method. Cells were treated with H₂O₂, and the medium of the control group was changed at the same time, and then, the medium of cells was collected with or without H₂O₂ treatment 2 h later, and MDA levels were detected. (c) NRK-52e cells were pretreated with 1 mmol/L NAC or 1 μmol/L fenofibrate for 1 h, followed by treatment with 100 μmol/L H₂O₂ for 2 h. DHE and Hoechst 33342 were incubated for 30 min, and a fluorescence microscope was used to observe the fluorescence intensity (red, DHE, exposure time (1.3 s); blue, Hoechst 33342, exposure time (70 ms)). (d) Quantitative analysis was conducted with Image-Pro Plus. , .

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