Fenofibrate Improved Interstitial Fibrosis of Renal Allograft through Inhibited Epithelial-Mesenchymal Transition Induced by Oxidative Stress

Figure 2

Oxidative stress-induced EMT in rat renal tubular epithelial cells. (a) NRK-52e cells were treated with 100 μmol/L H₂O₂ for 2 h and cultured for 24 h, and then Western blot was conducted to detect protein expression. (b) Quantitative analyses were conducted with ImageJ. (c) The cells were pretreated with 1 mM NAC, then treated with 100 μmol/L H₂O₂ for 2 h. The expression of N-cadherin, vimentin, S100A4, collagen I, Snail, and cleaved caspase-3 was detected by Western blot. (d) Quantitative analyses were conducted with ImageJ. , .

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