Lycorine Attenuates Autophagy in Osteoclasts via an Axis of mROS/TRPML1/TFEB to Reduce LPS-Induced Bone Loss

Figure 1

Lycorine protects against LPS-induced bone loss in mice. (a) Representative images and relative intensities of the distal femurs from X-ray radiograms of mice treated with PBS (), LPS (5 mg/kg/week) (), or LPS+lycorine (2.5 mg/kg/d, ; 4 mg/kg/d, ; 6 mg/kg/d, ) were measured using the ImageJ program. , , and compared with PBS-treated mice. ^# and ^### compared with LPS-treated mice. Similar results were obtained in three independent experiments. (b) Representative μCT images of distal femora 1.0 mm from the growth plate of mice treated with PBS (), LPS (5 mg/kg/week) (), LPS+lycorine (6 mg/kg/d) (), or lycorine only (6 mg/kg/d) (). Mice were treated for 3 weeks beginning at the age of 10 weeks. (c) To examine TRAP-positive OCs in vivo, mouse femora were excised, cleaned with a soft tissue, and decalcified in EDTA. Representative histological sections of the distal femoral metaphysis of mice from each of the 4 groups were stained for TRAP to identify OCs (indicated by arrows head) to calculate OC.N/BS (OC number divided by total bone surface) and OC.S/BS (OC surface area divided by total bone surface area). Scale bar: 20 μm in the representative photos. and compared with PBS-treated mice. ^# and ^### compared with LPS-treated mice. Differences between groups were analyzed by two-way ANOVA, followed by Bonferroni posttests to compare the effect of lycorine (OC.S/BS and OC.N/BS; ) and the effect of LPS (OC.N/BS; ) and interaction (OC.S/BS; ). Similar results were obtained in three independent experiments.

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