Resveratrol Inhibits MMP3 and MMP9 Expression and Secretion by Suppressing TLR4/NF-κB/STAT3 Activation in Ox-LDL-Treated HUVECs

Figure 1

Resveratrol inhibits TLR4 expression in ox-LDL- and LPS-treated HUVECs. The cells were treated with various concentrations of resveratrol for 1 h, and ox-LDL (0.1 mg/ml) or LPS (5 μg/ml) was then added for 8 h. TLR4 expression was analyzed by Western blot and immunofluorescence. The column chart represents the density analysis from three independent experiments. (a) Analysis of cell viability by various concentrations of resveratrol. (b) TLR4 expression that was induced by various concentrations of ox-LDL. (c) Resveratrol inhibited TLR4 expression that was induced by ox-LDL. (d) Resveratrol inhibited TLR4 expression that was induced by LPS. (e) TLR4 expression was determined by immunofluorescence. Resveratrol (100 μM) reduced TLR4 expression in ox-LDL-treated HUVECs. , significant difference between non-ox-LDL-treated cells and ox-LDL-treated cells; , , significant difference between non-resveratrol-treated cells and resveratrol-treated cells.

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