Resveratrol Inhibits MMP3 and MMP9 Expression and Secretion by Suppressing TLR4/NF-κB/STAT3 Activation in Ox-LDL-Treated HUVECs

Figure 5

Resveratrol inhibits NF-κB p65 and STAT3 phosphorylation and nuclear translocation in ox-LDL- and LPS-treated HUVECs. The cells were treated with various concentrations of resveratrol for 1 h, and ox-LDL (0.1 mg/ml) or LPS (5 μg/ml) was then added for 8 h. The phosphorylation of NF-κB and STAT3 was analyzed by Western blot. The column chart represents the density analysis from three independent experiments. (a, b) Resveratrol inhibited NF-κB p65 phosphorylation that was induced by ox-LDL and LPS. (c, d) Resveratrol inhibited STAT3 phosphorylation that was induced by ox-LDL and LPS. (e) Resveratrol reduced IL-6 secretion that was induced by ox-LDL. (f, g) Resveratrol inhibited NF-κB and STAT3 nuclear translocation that was induced by ox-LDL. , significant difference between non-ox-LDL-treated cells and ox-LDL-treated cells or between non-LPS-treated cells and LPS-treated cells; , , significant difference between non-resveratrol-treated cells and resveratrol-treated cells.

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