Anti-Inflammatory Effect of an Apigenin-Maillard Reaction Product in Macrophages and Macrophage-Endothelial Cocultures

Figure 5

DMA prevents AGE-induced macrophage adhesion to endothelial cells. HUVECs at the lower compartment of the Boyden chamber were incubated with medium (a), 500 μg/mL AGEs (b), (c), or (d) for 48 hrs. Macrophages adhered to HUVECs were stained by 0.1% crystal violet, and representative photos were taken under a microscope. (e) Macrophage counting. The stain was washed off by 30% acetic acid and measured at a wavelength of 580 nm. vs. control; ^### vs. AGE treatment.

(a)

(b)

(c)

(d)

(e)