Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 5

Research Article

Nω-(Carboxymethyl)arginine Is One of the Dominant Advanced Glycation End Products in Glycated Collagens and Mouse Tissues

Figure 5

Identification of CMA peptide. (a) MS/MS spectrum of ESI-QTRAP of the CMA peptide purified with immunoaffinity column chromatography. The only CMA peptide observed after immunoaffinity purification was identified as GAAGPOGPOGSAGTOGLQGMOGERGGOGGOGPK at 992.11 () (+, O represents hydroxyproline and R represents CMA). (b) The repetitive yield of amino acids in each step of the Edman degradation. The immunoaffinity-purified peptide was also analyzed using the 491 Protein Sequencer. The identified sequence was the same as that obtained by LC-MS/MS analysis, except for an unreadable 24th residue (asterisk).

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