Research Article
Tocotrienol-Rich Fraction (TRF) Treatment Promotes Proliferation Capacity of Stress-Induced Premature Senescence Myoblasts and Modulates the Renewal of Satellite Cells: Microarray Analysis
Table 1
Quantitative real-time PCR: (a) primer sequences and (b) the thermal cycling profile.
(a) |
| Accession number | Gene | Primer type | Primer sequences (5–3) |
| NM_001289746 | GAPDH | Forward | TTGCCCTCAACGACCACTTT | Reverse | TGGTCCAGGGGTCTTACTCC |
| NM_004864.2 | GDF15 | Forward | CAGGACGGTGAATGGCTCTC | Reverse | TAGCGTTTCCGCAACTCTCG |
| NM_001432.2 | EREG | Forward | CTGCAGGTGTGAAGTGGGTT | Reverse | GTGGAACCGACGACTGTGAT |
| NM_015713.4 | RRM2B | Forward | AGAGTTCTCGCCGGTTTGTC | Reverse | GTCGACCTCTTCTGCTGTCC |
| NM_016848.5 | SHC3 | Forward | GCTTTTGCTGTGAGCAGCC | Reverse | CCACCGTTAAAAGCCAGCAC |
| NM_183001.4 | SHC1 | Forward | CTTGGGAGCTACATTGCCTGT | Reverse | TCAAAAAGCTCTCTGCCTGGAC |
| NM_014454.2 | SESN1 | Forward | TTGACAGCTCCACAACGTGA | Reverse | TGTACACGAAAGGGCAGTCT |
| NM_005259.2 | MSTN | Forward | TTGACATGAACCCAGGCACT | Reverse | GTCCTGGGAAGGTTACAGCA |
| NM_002478.4 | MYOD1 | Forward | CGCCAGGATATGGAGCTACT | Reverse | GAGTGCTCTTCGGGTTTCAG |
| NM_005902.3 | SMAD3 | Forward | CATGTCGTCCATCCTGCCTT | Reverse | TTGGTGTTGACGTTCTGCGT |
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(b) |
| Cycling step | Temperature | Time | Number of cycles |
| Enzyme activation/initial DNA denaturation | 95°C | 30 seconds | 1 | Denaturation | 95°C | 5 seconds | 40 | Annealing/extension | 60°C | 30 seconds | Melt curve | 65°C to 95°C (in 0.5°C increments) | 5 seconds | 1 |
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