Tocotrienol-Rich Fraction (TRF) Treatment Promotes Proliferation Capacity of Stress-Induced Premature Senescence Myoblasts and Modulates the Renewal of Satellite Cells: Microarray Analysis

Table 1

Quantitative real-time PCR: (a) primer sequences and (b) the thermal cycling profile.

(a)

Accession number	Gene	Primer type	Primer sequences (5–3)
NM_001289746	GAPDH	Forward	TTGCCCTCAACGACCACTTT
		Reverse	TGGTCCAGGGGTCTTACTCC
NM_004864.2	GDF15	Forward	CAGGACGGTGAATGGCTCTC
		Reverse	TAGCGTTTCCGCAACTCTCG
NM_001432.2	EREG	Forward	CTGCAGGTGTGAAGTGGGTT
		Reverse	GTGGAACCGACGACTGTGAT
NM_015713.4	RRM2B	Forward	AGAGTTCTCGCCGGTTTGTC
		Reverse	GTCGACCTCTTCTGCTGTCC
NM_016848.5	SHC3	Forward	GCTTTTGCTGTGAGCAGCC
		Reverse	CCACCGTTAAAAGCCAGCAC
NM_183001.4	SHC1	Forward	CTTGGGAGCTACATTGCCTGT
		Reverse	TCAAAAAGCTCTCTGCCTGGAC
NM_014454.2	SESN1	Forward	TTGACAGCTCCACAACGTGA
		Reverse	TGTACACGAAAGGGCAGTCT
NM_005259.2	MSTN	Forward	TTGACATGAACCCAGGCACT
		Reverse	GTCCTGGGAAGGTTACAGCA
NM_002478.4	MYOD1	Forward	CGCCAGGATATGGAGCTACT
		Reverse	GAGTGCTCTTCGGGTTTCAG
NM_005902.3	SMAD3	Forward	CATGTCGTCCATCCTGCCTT
		Reverse	TTGGTGTTGACGTTCTGCGT

(b)

Cycling step	Temperature	Time	Number of cycles
Enzyme activation/initial DNA denaturation	95°C	30 seconds	1
Denaturation	95°C	5 seconds	40
Annealing/extension	60°C	30 seconds
Melt curve	65°C to 95°C (in 0.5°C increments)	5 seconds	1