Proximal Tubular Development Is Impaired with Downregulation of MAPK/ERK Signaling, HIF-1α, and Catalase by Hyperoxia Exposure in Neonatal Rats

Figure 6

Hyperoxia alters HIF-1α and catalase expression in S-shaped bodies. (a) Hypoxia-inducible factor 1α (HIF-1α) and catalase expression in S-shaped bodies of newborn rats exposed to normoxia or hyperoxia was detected by immunohistochemical staining (original magnification ×400; scale bar, 100 μm). (b) The immunostaining intensity of HIF-1α expression in S-shaped bodies exposed to normoxia or hyperoxia is shown by box and whisker plots. Relative expression is standardized to the value of the normoxia group on P1D. The whiskers represent the minimal intensity or the maximal intensity, and the boxes span the interquartile range of measurements for 10 rats and the mean value of 10 separate fields of view (). , one-way ANOVA, Bonferroni post hoc test. (c) The immunostaining intensity of catalase expression in S-shaped bodies exposed to normoxia or hyperoxia is shown by box and whisker plots. Relative expression is standardized to the value of the normoxia group on P1D. The whiskers represent the minimal intensity or the maximal intensity, and the boxes span the interquartile range of measurements for 10 rats and the mean value of 10 separate fields of view (). , one-way ANOVA, Bonferroni post hoc test.

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