Dihydrotanshinone-Induced NOX5 Activation Inhibits Breast Cancer Stem Cell through the ROS/Stat3 Signaling Pathway

Figure 1

Effect of DHTS on mammosphere formation and multiple cancer hallmarks in breast cancer cell lines. Effect of tanshinones on mammosphere formation. MCF-7 and MDA-231-MB cells were cultured in mammosphere-forming conditions for 7 days. Primary mammospheres were incubated with tanshinones (0, 1, 2.5, 5, 10, and 15 μM) or DMSO. Chemical structure of DHTS (a). Effect of DHTS on the viability of MCF-7 and MDA-MB-231 cells. The two breast cancer cells were treated with DHTS for 24 h. The antiproliferative effect of DHTS was measured by MTS assays (b). Effect of DHTS on mammosphere formation. The mammospheres were incubated with DHTS (0.5 and 1 μM) or DMSO. MCF-7 and MDA-MB-231 cells were treated with DHTS for 7 days in CSC culture media. Images were obtained by microscopy and are representative mammospheres (scale ) (c). Effect of DHTS on the migratory potential of human breast cancer cells. The wound healing of MDA-MB-231 cells with or without DHTS photographed at 0 and 18 h (scale ) (d). Effect of DHTS on colony formation of human breast cancer cells. One thousand dissociated MDA-231-MB cells were seeded in 6-well plates and treated with the indicated concentrations of DHTS for 7 days. Representative images of colonies were recorded (e). The data shown represent the of three independent experiments. vs. DMSO-treated control.

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